Steroid Hormones Library
Steroid Hormones DMPK and Bioanalytical Services
Steroid hormone bioanalysis introduces two enzyme classes not seen anywhere else in this library: 11β-hydroxysteroid dehydrogenase (11β-HSD) and deiodinases. Hydrocortisone and prednisone are 11β-HSD prodrugs — not CYP, not UGT, not esterase — requiring NAD+/NADP+ cofactor systems distinct from every other prodrug activation assay in this collection. Liothyronine (T3) is metabolized by deiodinases via halogen removal — a reaction mechanism unrelated to oxidation, reduction, hydrolysis, or glucuronidation. Beyond these two novel enzyme classes, the remaining five compounds span CYP3A4 metabolism (testosterone, progesterone), CYP3A4 induction + P-gp (dexamethasone), Phase I+II dual metabolism (estradiol), and non-metabolized excretion (prednisolone) — six distinct analytical approaches for eight compounds.
Creative Proteomics provides 11β-HSD prodrug activation monitoring, deiodinase metabolism profiling, CYP3A4 inducer+substrate DDI risk assessment, Phase I+II dual-cofactor profiling, and non-metabolized steroid hormone disposition.
11β-HSD Prodrug ActivationHydrocortisone and prednisone are activated by 11β-HSD — a first in this library. Requires NAD+/NADP+, not CYP or UGT cofactors.
Deiodinase: Halogen RemovalT3 metabolism via deiodinases is a novel enzyme class — not oxidation or glucuronidation.
Dexamethasone: CYP3A4 Inducer + P-gpCYP3A4 substrate AND inducer with P-gp transport — three analytical dimensions.
Steroid Enzyme Diversity DMPK Strategy Map
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11β-HSD Prodrug Activation (2 compounds)Hydrocortisone and prednisone require 11β-HSD with NAD+/NADP+ cofactor — the first appearance of this enzyme class in the library.
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CYP3A4 Inducer + Substrate + P-gp (Dexamethasone)CYP3A4 metabolism, CYP3A4 induction (accelerated co-substrate clearance), and P-gp transport — three DDI dimensions in one steroid.
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Deiodinase (T3) + Phase I+II (Estradiol)T3: halogen removal by deiodinases. Estradiol: CYP3A4/CYP1A2 Phase I + UGT Phase II — dual-cofactor required.
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CYP Phase I + Non-Metabolized (4 compounds)Testosterone/progesterone: CYP3A4 Phase I. Prednisolone: excreted unchanged. Shared CYP framework serves multiple steroids.
Steroid hormone enzyme audit before method development.Creative Proteomics maps each compound's enzyme system — 11β-HSD, deiodinase, CYP3A4, or none — because hydrocortisone needs NAD+, T3 needs deiodinase cofactors, and estradiol needs NADPH+UDPGA. Six analytical approaches, one class.
